In Vivo Evaluation of Oxygenic Photogranules’ Photosynthetic Capacity by Pulse Amplitude Modulation and Phototrophic–Irradiance Curves

The commingled microbial moiety of oxygenic photogranules (OPGs) facilitates aeration-free wastewater treatment. Embedded in an extracellular polymeric substances (EPS) matrix, producers and consumers oxygen occupying granular niches exchange substrates among themselves with the bulk fluid. An assessment OPG’s phototrophic potential or functional capacity may require combining different photoactivity signals. photosynthetic was evaluated using evolution (POE) chlorophyll fluorescence (rapid light curves, RLC) measurements OPGs grown at intensities.. A maximum generating for optimal reactor conditions determined to be 284.4 mgO2 gVSS–1 h–1 exhibited photoelasticity, higher high (HL) compared that low (LL) adapted samples. Saturation irradiances before onset photoinhibition LL HL samples were 1000 1200 ?mol m–2 s–1, respectively POE signals, 478 s–1 611 RLC Moreover, had nonphotochemical quenching rates which allude photoelastic potential. correlation coefficients (?) between RLCs lower than reported values pure cultures reflecting enhanced contribution from clades a variety light-harvesting pigments present OPGs. In OPG reactor, photochemical activity can influenced by size, ecology, operation metrics related irradiance interactions such as mixing, self-shading, intensity, photoperiods, depth. This presents opportunities design intensive resource recovery biomass.